Tirf, srrfstream, point localization superresolution. Microscope leica sp5 confocal user manual 65 pages microscope leica sp8 training manual. Leica vlux typ 114 camera user manual, instruction manual, user guide pdf free download leica vlux typ 114 pdf user manual, user guide, instructions, leica vlux typ 114 owners manual. Sp5 confocal microscope user manual, helene javot updated by mamta srivastava. Jun 09, 2015 leica application suite x las x is the easy to use software platform for advanced life science research on leica microsystems confocal, widefield and superresolution systems. National chiao tung university, bio photonics lab, bypraveen kumar. Downloads leica q leica q photography leica camera ag. Modify zoom and image size to get the best sampling of your image see page 27 16. View online or download leica tcs sp5 x user manual, manual. Leica tcs sp5 ii the only broadband confocal technical documentation living up to life. Imaging diverse fluorophores in fixed or live samples temperature, humidity, co2 control spectrally tunable detectors ultrafast resonant scanning reduced photodamage with live samples 3d zstacks, timelapse, multiposition timelapse frapflipphotoactivation, fret ratio. Typing your password identifies you as a valid user for this system. Service manuals karl kaps lamp control lp162 schematics 180 kb download karl kaps som42,52 service manual 2.
Microscopes and imaging systems leica microsystems. Leica application suite x las x is the one software platform for all leica microscopes. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. The configuration window is used to setup the system with respect to the excitation laser lines, beam paths and detection channels. To ensure that the national laws, regulations and conditions for the opera. Spectral detection in combination with the sensitivity of leica hyd hybrid. Leicadmi6000 user manual this manual by leica microsystems gives a detailed description of the dmi6000 inverted microscope base. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence.
This manual is the first section of a three part leica tcs sp5 user guide. From basic documentation to advanced life science research las x. Leica dm6000cs stand upright motorized scanning stage lasers. To maintain information on the most current level, the description of software functions was. It is recommended setting up a separate user id for each user set up by the system administrator. The main focus of this operating manual is directed to the safety notes that must be strictly adhered to when working with the leica tcs sp5 and leica tcs sp5 x. Conducting a crosstalk test using the leica tcs sp5 confocal microscope. The leica sp5 confocal is a dmi 6000 model inverted microscope with a super z galvo stage for fast z sectioning and prisms for dic imaging.
Theres no need to invest in a dedicated instrument to see lifes details. The leica tcs sp5 is supplied with the latest version of the licensed las af software. Rmbf leica sp5 aobs spectral confocal core facilities. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens.
Imaging diverse fluorophores in fixed or live samples temperature, humidity, co2 control spectrally tunable detectors ultrafast resonant scanning reduced photodamage with live samples 3d zstacks, timelapse, multiposition timelapse frapflipphotoactivation, fret ratio or acceptor photobleaching. Leica dm2500 upright fluorescent microscope manuals. Leicaflex and leica r manuals r3 r4 r5 r6 r7 r8 r9. To inform leica geosystems immediately if the product and the application becomes unsafe. To maintain information on the most current level, the. Leica sp8 upright confocal duke light microscopy core. Utilizing a workflowbased structure, the software is built to get you up and running. Read carefully through the user manual before you switch on the product.
Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc. The system provides the full range of scan speeds at the highest resolution. Leica stereozoom manuals and user guides, microscope. Brochures, firmware, technical data, instructions, images and more. Leica sp5 scanning confocal cdb equipment resource. Usermanual for leica sp8 confocal contact information. Leica am total internal reflection fluorescence manual. To be familiar with local regulations relating to safety and accident prevention.
This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. Tltransmitted light 2 toggles the shutter between open and closed for brightfield illumination. The leica sp5 has four laser lines 405,488458, 476, 496, 514 561, and 633nm and is capable of capturing 5 channels which includes a dic brightfield detector. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. March 2017 apes picoemerald a reference list introduction ramanbased microscopy and spectroscopy techniques allow samples to be investigated without labeling. Overview highspeed and highsensitivity multimodal confocal imaging with 2 pinhole sizes 25um and 40um four laser lines.
Instructions for the leica sp5 ii laser scanning confocal microscope. How to use leica confocal microscope sp5 external laser. Image window robarts research institute leica sp5 training manual september 2010. Leica dlux 5 camera user manual, instruction manual, user. Zeiss axio observer z1 manual single or tile imaging with zen. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. Pcic sp5 confocal microscope user manual, helene javot updated by mamta srivastava 2112017, page 116 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. The leica sp5 does not use emission filters to define the wavelengths of emission collected, but rather has. Widely recognized for optical precision and innovative technology, leica microsystems is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning and superresolution microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopy. Leica sp5 inverted confocal duke light microscopy core facility. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of.
Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix checkin and start up. Jul 16, 2016 the leica r5 and re have an electronicallytimed shutter with verticaltraveling metal blades. Trademarks throughout this manual, trademarked names may be used. Andor dragonfly spinning disk confocal microscope users. Purchase congratulations on the purchase of a leica na720724728730730 plus. In addition, a uv diode laser provides excitation at 405 nm thus enabling the imaging of dyes such as dapi and the blue fluorescent proteins. Leica sp5 ii confocal user guide university of missouri. Overview on general stepsoverview on general steps page 3 1. All rights to this documentation are held by leica microsystems cms. Leica dm2500 upright fluorescent microscope manuals cell. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for stitching numerous fields into one large image.
View and download leica sp5 confocal user manual online. Leica tcs sp5 confocal laser scanning microscope user guide. Maximum shutter speed was increased compared with leica s previous models. Sequential scanning using the leica tcs sp5 confocal laser scanning microscope this supplemental manual is the second section of a three part leica tcs sp5 user guide edited by donald pottle schepens 149 w dwp mar. Ladapter leica accessories lmount ssystem cameras ssystem lenses slsystem cameras slsystem lenses msystem cameras msystem lenses msystem equipment q camera. This confocal is reserved for primary and joint cdb faculty and their personnel. Leica application and technology support group, life science division. An introduction from your bosssupervisor is not enough the system has 5 detectors. User manual related manuals leica m680 surgical microscope leica m525 mc1 versatile surgical microscope.
Leica microsystems heidelberg gmbh shall not be responsible for any direct, incidental or consequential damages arising from the use of any material contained in this document. Consultation with the equipment manager is required before using this microscope. The information contained in this operating manual is subject to change without prior notice. Only people who have had an introduction from kees straatman are allowed to operate the system. Leica application suite x microscope software youtube.
Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. Move in the z direction to find your plane of interest 15. Leica sp5 inverted confocal duke light microscopy core. Quick operation manual for leica sp5 confocalmultiphoton. Leica am total internal reflection fluorescence manual leica sp5 inverted confocal manual live cell station. To ensure that it is used in accordance with the instructions. Leica dmi6000cs inverted live cell chamber ludin cube metal halide fluorescence source motorized scanning stage tandem scanners select at software initialization conventional scanner resonant scanner 8 000 hz lasers. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems.
Slide the red locking mechanism to the side and open the battery compartment. This screen highlights the aperture opening and the intensity of the transmitted light. Confocal microscopes light microscopy core facility. Saving your work using the leica tcs sp5 confocal microscope system. Starting the lcs software on the leica tcs sp5 confocal microscope. Leica microsystems cms gmbh reserves the right to revise this. Leica biosystems offers products for each histology step and highproductivity workflow solutions for the entire. The microscope is an upright dm6000 and has a motorized stage.
Leica microsystems is a world leader in providing innovative microscopy, camera and software solutions for imaging and analysis of macro, micro and nanostructures. Mar 04, 2015 panasonic lumix dmclx5 camera user manual, instruction manual, user guide pdf canon powershot sd4000 is ixus 300 hs ixy 30s camera user manual, instruction manual, user guide pdf leica dlux 4 camera user manual, instruction manual, user guide pdf. Leica application and technology support group, life science division exton, pa 19341, usa leica tcs sp5 lasaf new user guide. Almost 500,000 rmount bodies were produced and sold since the leicaflex was introduced in 1964. Leica application suite x las x is the imaging and analysis software for live cell imaging from basic documentation to advanced fluorescence imaging, including superresolution and confocal microscopy. The technique visualizes structures in samples by detecting the.
The leica sp5 confocal system has eight visible laser lines provided by a multiline argon laser, a diodepumped solid state laser, and orange and red helium neon lasers. Leica tcs sp5 user manual indiana university bloomington. Leica tcs sp5 user manual this manual by leica micorsystems explains theory and background of the functionality of the sp5 and thus is a good complementation of the above stepbystep manual. Database contains 1 leica stereozoom manuals available for free online viewing or downloading in pdf. Leica microsystems heidelberg gmbh assumes no responsibility for any errors or omissions. The new leica tcs sp8 sted one 592 and sted one 660 nanoscopes are your strategic entry into nanoscopy on the basis of the highly versatile innovative confocal platform leica tcs sp8. Remove the locking clip and slide the endpiece down. The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs sp5 delivers bright, noisefree. Microscope manuals are presented in a printable format, with information divided into functional sections for ease of access.
Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Equipment manuals duke light microscopy core facility. Leica tcs sp5 confocal louisiana state university health. Two of these detectors are very sensitive hyd sp gaaspdetectors. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions. Accessories make your camera outfit even more user friendly. The rcameras used to be the flagships in leica s photographic equipment portfolio and a popular choice for professional and enthusiast photographers alike. Turning on the confocal system instructions are posted in the room 1. User manuals, guides and specifications for your leica stereozoom microscope. Leica four independent companies share the leica brand. Click on qlut to display the image with false colors blue points are saturated pixels 255 level and green points are thresholded pixels 0 level.
With motorized zfocus, motorized and coded 7x nosepiece, fully automated transmitted light axis, and fully automated 5x or 8x fluorescence axis, the automated leica dm6000 b research microscope provides all transmitted light contrast methods including the worlds first fully automated differential interference contrast dic unique is the ability to recall a particular contrast method. Leica tcs sp5 confocal louisiana state university health sciences centershreveport. Shutter speeds vary in fullstop intervals from 12 second to 12000 seconds in m and t modes, and are continuouslyvariable from 15 seconds to 12000 seconds in a and p modes. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. View and download leica tcs sp5 user manual online.